中山大学附属第七医院科研中心
Research Center, The Seventh Affiliated Hospital of Sun Yat-sen University, Guangming, Shenzhen
Email: yingw3@mail.sysu.edu.cn
研究兴趣/Research
课题组位于中山大学深圳校区医学园，通过综合应用生物物理与生物化学研究方法，揭示疾病相关靶点蛋白质的复杂机制。基于对蛋白质构象与动态的深入解析研究新的治疗与诊断策略。当前研究方向包括肿瘤与遗传病中的KRAS突变、神经递质-钠同向转运体(NSS)的药物作用机制以及NSS在神经退行性疾病淀粉样病变中的调控作用、GPCR靶向药物筛选策略、靶向蛋白降解机制等。
Our lab is lcoated in the Medical Building on Shenzhen Campus of Sun Yat-sen University. We are dedicated to uncovering the molecular mechanisms of disease-relevant, challenging protein targets through an integrated approach combining biophysical and biochemical methods. Guided by insights into protein structure and dynamics, our goal is to develop innovative therapeutic and diagnostic strategies. Our current research focuses on:
1. KRAS mutations in tumors and genetic disorders – We focus on both hotspot and rare KRAS mutations, investigating novel tumor-suppressive mechanisms involving RAS proteins and developing targeted therapeutic strategies.
2. Neurotransmitter:sodium symporters (NSSs) – We study the structural mechanisms of drug actions on NSSs and explore the potential role of NSS in modulating amyloidiosis associated with neurodegenerative diseases.
3. GPCR ligand discovery – We are developing innovative screening strategies to identify allosteric ligands targeting G protein-coupled receptors.
4. Targeted Protein Degradation – We investigate the dysregulation of proteolytic pathways under pathological conditions and explore their implications for disease progression and therapeutic opportunities.
Our lab actively engages in cutting-edge collaborations and is committed to translating biological research into clinical applications to address unmet medical needs.
教育与学术经历/Education and Accademic Experience
学士 西安交通大学/BSc., Chemistry, Xi’an Jiaotong University;
硕士 中国人民大学/MS, Chemistry, Renmin University of China;
博士 哥廷根大学/马克斯普朗克研究所/Dr. rer. nat., Biology, University of Göttingen/Max-Planck-Institute for Biophysical Chemistry (current MPI-Multidisciplinary Sciences);
博士后 北卡大学教堂山分校医学院/Postdoc., Department of biochemistry and biophysics, School of Medicine, the University of North Carolina at Chape Hill;
研究员 哥伦比亚大学医学院/Associate Research Scientist, Columbia University Irving Medical Center
近期研究论文/Recent Pubilcations
[1] Ajit Prakash, Zijian Li, Venkat Chirasani, Juhi Rasquinha, Natalie Hewitt, Garrett Hubbard, Guowei Yin, Aspen Hawkins, Luca Montore, Henrik Dohlman, Sharon Campbell, Molecular and functional profiling of Gαi as an intracellular pH sensor, Nat Commun, 2025, 16:3468.
[2] Qi Liang , Haiyue Zuo, Tian Yang, Jiatai Yin, Xiaomin Huang, Jing Wang, Qian Li, Guowei Yin*, Xinfeng Zhao*, Discovery of dual-target ligands binding to beta2-adrenoceptor and cysteinyl-leukotriene receptor for the potential treatment of asthma from natural products derived DNA-encoded library, Eur. J. Med. Chem., 2022, 233:114212.
[3] Guowei Yin*, Jing Huang, Johnny Petela, Hongmei Jiang, Yuetong Zhang, Siqi Gong, Jiaxin Wu, Bei Liu, Jianyou Shi, Yijun Gao, Targeting small GTPases: emerging grasps on previously untamable targets, pioneered by KRAS, Signal Transduct Target Ther., 2023, 8:212.
[4] Guowei Yin*, Guohua Lv, Jerry Zhang, Hongmei Jiang, Tianqi Lai, Yushan Yang, Yong Ren, Jing Wang, Chenju Yi, Hao Chen, Yun Huang, Chaoni Xiao, Early-stage structure-based drug discovery for small GTPases by NMR spectroscopy, Pharmacol. Ther., 2022, 236:108110.
[5] Qian Li#, Guowei Yin#, Jing Wang, Linkang Li, Qi Liang, Xue Zhao, Yuanyuan Chen, Xiaohui Zheng, Xinfeng Zhao, An emerging paradigm to develop analytical methods based on immobilized transmembrane proteins and its applications in drug discovery, Trends in Analytical Chemistry, 2022, 157: 116728.
[6] Kevin Knight, Soumadwip Ghosh, Sharon Campbell, Tyler Lefevre, Reid Olsen, Alan Smrcka, Natalie Valentin, Guowei Yin, Nagarajan Vaidehi, and Henrik G. Dohlman, A Universal allosteric mechanism for G protein activation, Mol. Cell, 2021, 81:1-13.
[7] Vinay Nair, Guowei Yin, Jerry Zhang, John Hancock, Sharon Campbell, Alemayehu A Gorfe, Monoubiquitination of KRAS at lysine104 and lysine147 Modulates Its dynamics and interaction with partner proteins, J. Phys. Chem. B, 2021, 125: 4681-4691.
[8] Guowei Yin#, Jerry Zhang#, Vinay Nair, Vinh Truong, Angelo Chaia, Johnny Petela, Joseph Harrison, Alemayehu Gorfe, Sharon Campbell, KRAS ubiquitination at lysine 104 retains exchange factor regulation by dynamically modulating the conformation of the interface, iScience, 2020, 23:101448.
